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avidin biotin complex abc peroxidase reagent  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin complex abc peroxidase reagent
    Avidin Biotin Complex Abc Peroxidase Reagent, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+complex+abc+peroxidase+reagent/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13061616-236-13-23
    Average 99 stars, based on 12720 article reviews
    avidin biotin complex abc peroxidase reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Astrocytes enable amygdala neural representations supporting memory.
    Article Snippet: The sections were incubated overnight in mouse anti-GFP (A-11120, 1859591; 1:500, Invitrogen) in PBS with 1% BSA at room temperature, rinsed in PBS and incubated for 30 min in biotinylated goat anti-mouse antibody (31802, 1:200, Invitrogen). .. After rinsing in PBS, the sections were incubated for 30 min with an avidin/biotin complex (ABC) peroxidase reagent (Vectastain Elite ABC Kit PK-6100, Vector Labs) according to the manufacturer’s instructions, rinsed in PBS and reacted for 5 min with 1 mM 3,3-diaminobenzidine and 0.0015% hydrogen peroxide in PBS. ..

    Article Title: Astrocytes enable amygdala neural representations supporting memory
    Article Snippet: The sections were incubated overnight in mouse anti-GFP (A-11120, 1859591; 1:500, Invitrogen) in PBS with 1% BSA at room temperature, rinsed in PBS and incubated for 30 min in biotinylated goat anti-mouse antibody (31802, 1:200, Invitrogen). .. After rinsing in PBS, the sections were incubated for 30 min with an avidin/biotin complex (ABC) peroxidase reagent (Vectastain Elite ABC Kit PK-6100, Vector Labs) according to the manufacturer’s instructions, rinsed in PBS and reacted for 5 min with 1 mM 3,3-diaminobenzidine and 0.0015% hydrogen peroxide in PBS. .. After rinsing, the sections were incubated in 1% hydrogen peroxide in PBS for 15 min to quench the remaining peroxidase, then a second round of labelling was performed as above with rabbit anti-DsRed primary antibody (632496, 1904182; 1:500, Takara Bio), goat anti-rabbit secondary antibody (A32731, 1:200, Invitrogen) and ABC.

    Avidin-Biotin Assay:

    Article Title: Astrocytes enable amygdala neural representations supporting memory.
    Article Snippet: The sections were incubated overnight in mouse anti-GFP (A-11120, 1859591; 1:500, Invitrogen) in PBS with 1% BSA at room temperature, rinsed in PBS and incubated for 30 min in biotinylated goat anti-mouse antibody (31802, 1:200, Invitrogen). .. After rinsing in PBS, the sections were incubated for 30 min with an avidin/biotin complex (ABC) peroxidase reagent (Vectastain Elite ABC Kit PK-6100, Vector Labs) according to the manufacturer’s instructions, rinsed in PBS and reacted for 5 min with 1 mM 3,3-diaminobenzidine and 0.0015% hydrogen peroxide in PBS. ..

    Article Title: Astrocytes enable amygdala neural representations supporting memory
    Article Snippet: The sections were incubated overnight in mouse anti-GFP (A-11120, 1859591; 1:500, Invitrogen) in PBS with 1% BSA at room temperature, rinsed in PBS and incubated for 30 min in biotinylated goat anti-mouse antibody (31802, 1:200, Invitrogen). .. After rinsing in PBS, the sections were incubated for 30 min with an avidin/biotin complex (ABC) peroxidase reagent (Vectastain Elite ABC Kit PK-6100, Vector Labs) according to the manufacturer’s instructions, rinsed in PBS and reacted for 5 min with 1 mM 3,3-diaminobenzidine and 0.0015% hydrogen peroxide in PBS. .. After rinsing, the sections were incubated in 1% hydrogen peroxide in PBS for 15 min to quench the remaining peroxidase, then a second round of labelling was performed as above with rabbit anti-DsRed primary antibody (632496, 1904182; 1:500, Takara Bio), goat anti-rabbit secondary antibody (A32731, 1:200, Invitrogen) and ABC.



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